tumor necrosis factor alpha tnf α  (Boster Bio)

Journal: Materials Today Bio

Article Title: Immediate tumor killing and long-term anti-tumor immunoreaction induced by Bufalin-loaded phototherapeutic Janus membrane in CRC postoperative therapy

doi: 10.1016/j.mtbio.2026.102824

Figure Lengend Snippet: In vivo study in anti-tumor immune response by analysizing the maturation, activation and infiltration of immune-related cells, as well as the level of immune-related cytokines. (A) The maturation of DCs in lymph nodes of mice; (B) The activation of CD4 + /CD8 + T cells in spleens in of mice; (C) The activation of NK cells from spleens of mice. (D–G) The quantitative analysis of flow cytometry, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001. (H) Immunofluorescence photos of CD4 + and CD8 + T cell infiltrating tumor tissues of mice, bar: 100 μm. (I–K) The quantitative analysis of immune-related cytokines: IL-6, TNF-α and IFN-γ, ∗ p < 0.5, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001.

Article Snippet: The IFN-γ and TNF-α detection kit was purchased from Biodragon Biotechnology (Beijing), the IL-6 detection kit was obtained from BOSTER Biotechnology (Wuhan).

Techniques: In Vivo, Activation Assay, Flow Cytometry, Immunofluorescence

Journal: Materials Today Bio

Article Title: Immediate tumor killing and long-term anti-tumor immunoreaction induced by Bufalin-loaded phototherapeutic Janus membrane in CRC postoperative therapy

doi: 10.1016/j.mtbio.2026.102824

Figure Lengend Snippet: In vivo anti-tumor immune mechanisim analysis of BU-CDs CA-HA -loaded Janus membrane under NIR: (A–I) Flow cytometry and quantitative analysis of DCs, CD4 + /CD8 + T cells, NK cells and MDSCs, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; (J) Immunofluorescent staining of CD4 + /CD8 + T cells in tumor tissues to illustrate the enhanced tumor-specific identification and infiltration of T cells, bar: 50 μm; (K–L) Elisa test of proinflammatory cytokin (IL-6, TNF-α and IFN-γ) to assess the inflammatory activity, ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The IFN-γ and TNF-α detection kit was purchased from Biodragon Biotechnology (Beijing), the IL-6 detection kit was obtained from BOSTER Biotechnology (Wuhan).

Techniques: In Vivo, Membrane, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay

Journal: Materials Today Bio

Article Title: Ultrasound-responsive hydrogel with ROS scavenging and NO controllable release capabilities for accelerated healing of diabetic wounds

doi: 10.1016/j.mtbio.2026.102796

Figure Lengend Snippet: Immunofluorescence and ELISA analysis of diabetic wound areas. Immunofluorescence staining images of CD31 in diabetic wound skin tissue on day 21 (A) and CD31 + neovascularization density (B). Immunofluorescence staining images of VEGF in diabetic wound skin tissue on day 21 (C) and the relative protein expression of VEGF (D). Immunofluorescence staining images of ROS in diabetic wound skin tissue on day 21 (E) and the relative protein expression of ROS (F). Immunofluorescence staining images of CD86 and CD206 in diabetic wound skin tissue on day 21 (G), and the corresponding ratio (H). ELISA analysis of TNF-α (I), IL-6 (J), and TGF-β (K) in diabetic wound skin tissue on day 21. (US parameters, 1 MHz, 0.7 W cm −2 ); n = 3, ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001.

Article Snippet: ELISA kits for IL-6, TNF-α, and TGF-β, were supplied by BOSTER Biological Technology Co., Ltd. Primary antibodies against CD31 and VEGF, along with corresponding fluorescent secondary antibodies, were obtained from Abcam.

Techniques: Immunofluorescence, Enzyme-linked Immunosorbent Assay, Staining, Expressing